Strains or plasmids

Phenotypes and correlative characters

Sources

Bacterial strains

E. coli DH5α

φ80 LacZ△M15, deoR, recA1, endA1, hsdR17, supE, thi, gyrA, relA

This lad

CGMCC1.10327

L. mesenteroides

This lad

Leu ack::Tet^R

CGMCC1.10327 with ack knock-out

This work

Plasmids

pBR322

Tet^R

This lad

pTA2-T

T-Vector, 2.98kb, Amp^R, lacZ

TOYOBO

pTA2-ack

pTA2 containing ack fragment from L. mesenteroides

This work

pTA2-Tet

pTA2 containing Tet^R fragment from pBR322

This work

pTA2-T-U

pTA2-Tet containing ack upstream fragment from pTA2-ack

This work

pTA2-T-U-D

pTA2-T-U containing ack downstream fragment from pTA2-ack

This work